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Collection Journal of applied phycology
- Editeur : Springer
- ISSN : 0921-8971
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Seasonal phenology and metabolomics of the introduced red macroalga Gracilaria vermiculophylla, monitored in the Bay of Brest (France)
article dans des revues - 2017-02-08
Surget, Gwladys, et al.
Titre Seasonal phenology and metabolomics of the introduced red macroalga Gracilaria vermiculophylla, monitored in the Bay of Brest (France) Type d'Ã©tude article dans des revues Auteurs personnes Surget Gwladys Auteur Lann Klervi Le Auteur Delebecq Gaspard Auteur Kervarec Nelly Auteur Donval Anne Auteur Poullaouec Marie-Aude Auteur Bihannic Isabelle Auteur Poupart Nathalie Auteur Stiger-Pouvreau ValÃ©rie Auteur Date de publication 2017-02-08 Editeur(s) [S.l.] : Springer Journal of applied phycology, ISSN 0921-8971 RÃ©sumÃ© Seaweeds represent one of the largest groups of marine aliens in Europe and constitute a large percentage of all introduced marine species. In Brittany, the red macroalga Gracilaria vermiculophylla has invaded the bare areas of brackish waters in saltmarshes. In the Bay of Brest, the alga forms dense monospecific mats on the mud surface and occupies an empty ecological niche, in association with the invasive halophyte, Spartina alterniflora. The phenology of G. vermiculophylla was studied through seasonal monitoring of biomass, density and size of fragments, complemented by metabolomic monitoring using 1H HR-MAS NMR chemical footprinting analyses. Moreover, lipids and pigments were quantified, using high-performance thin layer chromatography for the former and high-performance liquid chromatography and spectrophotometry for the latter. This rhodophyte is present throughout the year, never fixed to a substrate on the mud, with a maximum biomass in the summertime. Phenological observations on algal populations demonstrated a high capacity for fragmentation, with a majority of fragments shorter than 3 cm. Metabolomic analyses highlighted a temporal variability of lipids, pigments and osmolytes between seasons. These results, combined with ecological data, improve our understanding of the acclimation of G. vermiculophylla in Brittany, where it is mainly present in a vegetative state throughout the year. Our study represents an important contribution to understanding the ecological strategies used by this invasive seaweed to colonize and persist in the Bay of Brest. CatÃ©gories thÃ©matiques alguepeuplement (biologique)introduction d'espÃ¨ces vÃ©gÃ©tales Ã©trangÃ¨resprolifÃ©ration d'alguesmarÃ©e rouge Mots-clÃ©s libres algue invasive/algues/algues invasives/Gracilaria vermiculophylla/HR-MAS NMR/Invasive seaweed/Lipid class/Osmolyte/plante invasives/Plantes invasives/Population biology/rade de Brest Echelle de territoire rÃ©gion (ou supra) Note DOI = 10.1007/s10811-017-1060-3
Langue(s) Anglais Format pp1-16 Document en ligne http://link.springer.com/article/10.1007/s10811-017-1060-3 Localisation du document AccÃ¨s au texte intÃ©gral rÃ©servÃ© sur Springer NÂ° d'Ã©dition (ISBN, DOI,...) 10.1007/s10811-017-1060-3 Permalink http://etudes.bretagne-environnement.org/index.php?lvl=notice_display&id=159923Biochemical and antiviral activities of enzymatic hydrolysates from different invasive French seaweeds
article scientifique - 2013-11
Hardouin, K., et al.
Titre Biochemical and antiviral activities of enzymatic hydrolysates from different invasive French seaweeds Type d'Ã©tude article scientifique Auteurs personnes Hardouin K. Auteur Burlot A-S Auteur Umami A. Auteur Tanniou A. Auteur Stiger-Pouvreau V. Auteur Wodiwati I. Auteur Bedoux G. Auteur Bourgougnon N. Auteur Date de publication 2013-11 Editeur(s) [S.l.] : Springer Revue scientifique Journal of applied phycology, ISSN 0921-8971 RÃ©sumÃ© Proliferations of green, brown and red algae appear in shallow sandy bays in North Brittany (France), and they represent a real economic constraint for the affected communities. In addition to the nuisance for residents and tourist activity, the communities must carry out systematic collection. The collected algae are spread on agricultural land spreading or composted, but these solutions reach their limits rapidly, bringing little added value to the collected algae. Seaweeds are potentially excellent sources of bioactive metabolites that could represent useful leads in the development of new functional ingredients in pharmaceutical and cosmetic industries. The aim of this study was to propose the use of an enzyme-assisted extraction as a tool to improve the extraction efficiency of antiviral compounds from three invasive French seaweeds. We selected the red Solieria chordalis, the green Ulva sp. and the brown Sargassum muticum as models for these experiments. In comparison with water extraction at 50 Â°C for the same time of treatment, enzymatic hydrolysis increased the yields. The data suggest the potential of enzymatic hydrolysis for producing active fractions in the function of the algal biomass, the behaviour of the cell wall, the selectivity and the action of the enzyme. Enzymatic hydrolysis appeared less effective for polyphenol recovery, but was a promising softer technique for recovering proteins, neutral sugars, uronic acids and sulphate groups. The solvent-free process, higher extraction rate and higher yields, coupled to time-saving and lower cost, make this method economical and sustainable. By using a cell viability assay, all hydrolysate fractions tested were shown to be non-toxic to Vero cells. After 3 days of treatment, no microscopically visible alteration of normal cell morphology was observed even at 500 Î¼g mLâˆ’1. S. chordalis extracts have an effective antiviral activity with EC50 between 23.0 and 101.1 Î¼g mLâˆ’1 at a multiplicity of infection of 0.001 ID50/cells; 100 % and 98 % cellular protection were obtained for 500 Î¼g mLâˆ’1 of hydrolysate extracts carbohydrase C3 and blank, respectively. Other extracts from S. chordalis inhibited viral production less effectively. CatÃ©gories thÃ©matiques alguemarÃ©e verte Echelle de territoire rÃ©gion (ou supra) Langue(s) FranÃ§ais Document en ligne http://link.springer.com/article/10.1007/s10811-013-0201-6# NÂ° d'Ã©dition (ISBN, DOI,...) 10.1007/s10811-013-0201-6 Permalink http://etudes.bretagne-environnement.org/index.php?lvl=notice_display&id=13863